Selection of Single-Domain Antibodies towards Western Equine Encephalitis Virus

Human-like antibodies neutralizing Western equine encephalitis virus

This study describes the development of the first neutralizing antibodies against Western equine encephalitis virus (WEEV), a member of the genus Alphavirus. WEEV is transmitted by mosquitoes and can spread to the human central nervous system, causing symptoms ranging from mild febrile reactions to life-threatening encephalitis. WEEV has been classified as a biological warfare agent by the US C...

Properties of monoclonal antibodies against glycoproteins of western equine encephalitis virus.

To analyze the biological activities of the alphavirus glycoproteins, eight different monoclonal antibodies against the two glycoproteins of western equine encephalitis virus were isolated. Five of the eight monoclonal antibodies were shown to be specific for E1 and three for E2 protein by an enzyme-linked immunosorbent assay and by radioimmunoprecipitation. Three of the five anti-E1 and all of...

Eastern, Western and Venezuelan Equine Encephalomyelitis Sleeping Sickness Eastern Equine Encephalomyelitis (EEE), Eastern Equine Encephalitis, Eastern Encephalitis Western Equine Encephalomyelitis (WEE), Western Equine Encephalitis Venezuelan Equine Encephalomyelitis (VEE), Peste Loca, Venezuelan Equine Encephalitis, Venezuelan Encephalitis, Venezuelan Equine Fever

Eastern equine encephalomyelitis (EEE), western equine encephalomyelitis (WEE), and Venezuelan equine encephalomyelitis (VEE) viruses are mosquito-borne pathogens that can cause nonspecific illnesses and encephalitis in equids (horses, mules, burros, donkeys and zebras) and humans in the Americas. Some of these viruses also affect birds and occasionally other mammals. No specific treatment is a...

Selection, characterization, and thermal stabilization of llama single domain antibodies towards Ebola virus glycoprotein

BACKGROUND A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered to enhance their properties. In this study, we generated sdAbs specific for E...

Eastern Equine Encephalitis Virus